"Daisy" aired only once, during a September 7, 1964, telecast of David and Bathsheba on The NBC Monday Night Movie. Johnson's campaign was widely criticized for using the prospect of nuclear war, as well as for the implication that Goldwater would start one, to frighten voters. The ad was immediately pulled, but the point was made. The commercial was rebroadcast over and over for free as it appeared on nightly news and on conversation programs in its entirety (remember there were only three TV channels at the time).
Daisy wasn't the only nuclear proliferation ad run by LBJ, there was also one called "Girl With An Ice Cream Cone."
The issue behind both commercials is that Johnson promised to wind down the Vietnam war, Goldwater wanted to ramp it up. Ironically Johnson did what Goldwater wanted to do, ramp up the war.
Remember how people were slaughtered for suggesting that Reverend Wright should be an issue in the 2008 campaign or even terrorist/Obama friend Bill Ayers? You haven't seen "guilt by association until you've seen the spot below. It was called, "KKK for Goldwater", and it portrayed Goldwater as being racist, by noting that Alabama KKK leader Robert Creel supported him. It didn't matter that Goldwater never met the guy.
Today's campaign spots can't hold a candle to the nastiness of what went on in 1964.